- Collection:
- Enhancing Global Research and Education in STEM at Spelman College (G-STEM)
- Title:
- The influence of dietary lipid composition on autophagy and apoptosis markers in skeletal muscle from mice following calorie restriction, 2014
- Creator:
- Kirkland, Faith
Villalba, Manuel
Oliver, Tiffany - Date of Original:
- 2014
- Subject:
- Science
Technology--Study and teaching - Location:
- United States, 39.76, -98.5
United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798 - Medium:
- abstracts (summaries)
- Type:
- Text
- Format:
- application/pdf
- Description:
- Aging in muscular tissue is associated with sarcopenia, an age-related loss of muscle mass and function. The goal of this study is to examine the effects of calorie restriction and lipid composition on apoptosis and autophagy markers in muscle. In order to achieve this aim, 64 male mice were assigned to fours groups based on diet: as libitum-soy, calorie restriction-lard, calorie restriction-soy and calorie restriction-fish oil. These groups were fed experimental diets for 6 or 18 months and then sacrificed. Muscle tissue was taken and mitochondrial and total homogenate fractions were analyzed for p16, Bax, LC3, BENCN1, and mTOR expression. The results indicated a trend towards lower levels of p16 expression under calorie restriction. The calorie restriction-lard group had a significantly higher expression of p16 than the calorie restriction-soy and -fish groups. There was no significant difference shown between soy ad libitum and calorie restriction for mTOR phosphorylation. Mitochondrial levels of Bax expression were significantly lower in calorie restriction group. Based on these findings, it could be concluded that the polyunsaturated fatty acid composition of lard effected p16 expression in muscle tissue. Also calorie restriction was shown to lower the levels of Bax in the mitochondrial fractions of muscle. This was novel information, because the evaluation of Bax in these diets had only been previously done in total homogenates.
- External Identifiers:
- Metadata URL:
- http://hdl.handle.net/20.500.12322/sc.gstem:2014_kirkland_faith
- Language:
- eng
- Extent:
- 1 page
- Original Collection:
- Enhancing Global Research and Education in STEM at Spelman College (G-STEM)
- Holding Institution:
- Spelman College
- Rights:
-
