- Collection:
- Spelman College Faculty Publications
- Title:
- Draft Genome of Cochliopodium minus (Amoebozoa): Insights into Its Complex Sexual Behavior, Across Domain Gene Acquisitions and Metazoan Type Signaling
- Creator:
- Tekle, Yonas I., Spelman College
Wang, Fang, Spelman College
Tran, Hanh, Spelman College
Hayes, T. Danielle, University of Florida
Ryan, Joseph F., University of Florida - Date of Original:
- 2021-12-23
- Subject:
- Spelman College--Faculty
African American scholars
African Americans--Education (Higher)--Georgia
African American universities and colleges--Georgia--Atlanta - Location:
- United States, Georgia, Atlanta Metropolitan Area, 33.8498, 84.4383
- Medium:
- articles
- Type:
- Text
- Format:
- application/pdf
- Description:
- To date, genomic analyses in amoebozoans have been mostly limited to model organisms or medically important lineages. Consequently, the vast diversity of Amoebozoa genomes remain unexplored. A draft genome of Cochliopodium minus, an amoeba characterized by extensive cellular and nuclear fusions, is presented. C. minus has been a subject of recent investigation for its unusual sexual behavior. Cochliopodium's sexual activity occurs during vegetative stage making it an ideal model for studying sexual development, which is sorely lacking in the group. Here we generate a C. minus draft genome assembly. From this genome, we detect a substantial number of lateral gene transfer (LGT) instances from bacteria (15%), archaea (0.9%) and viruses (0.7%) the majority of which are detected in our transcriptome data. We identify the complete meiosis toolkit genes in the C. minus genome, as well as the absence of several key genes involved in plasmogamy and karyogamy. Comparative genomics of amoebozoans reveals variation in sexual mechanism exist in the group. Similar to complex eukaryotes, C. minus (some amoebae) possesses Tyrosine kinases and duplicate copies of SPO11. We report a first example of alternative splicing in a key meiosis gene and draw important insights on molecular mechanism of sex in C. minus using genomic and transcriptomic data.
- External Identifiers:
- Metadata URL:
- http://hdl.handle.net/20.500.12322/sc.fac.pubs:2022_tekle_yonas_i_1
- Rights Holder:
- Spelman College
- Original Collection:
- bioRXiv Preprints
- Holding Institution:
- Spelman College
- Rights:
-
