- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- Recombinant antibodies against prostate cancer antigens, 2006
- Creator:
- Flowe, Candice M.
- Date of Original:
- 2006-05-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
- Type:
- Text
- Format:
- application/pdf
- Description:
- Efforts to identify tumor-associated antigens have historically involved monoclonal antibodies (MAbs) derived from hybridomas produced through the immunization of mice with human antigens or live tumor cells. The major disadvantage of this approach is that hybridoma cell-derived antibodies have either recognized tumor cells and normal cells, or identified intracytoplasmic antigens. This study will attempt to replace conventional methods by using a combination of selective and subtractive procedures using antibody-phage-display. This thesis has focused on the development of methods and their applications for the identification of antibodies reactive with tumor associated antigens (TAA). A semisynthetic single chain variable fragment library was screened to identify clones that bind preferentially to tumorigenic cells. The library was first subtracted on normal cells, then selected on tumorigenic cells. As a result, one clone was found to be tumor cell-specific. This thesis gives information on certain parameters that should be considered when developing methods for isolating tumor-specific clones through the use of phage display.
- External Identifiers:
- Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:2006_flowe_candice_m
- Rights Holder:
- Clark Atlanta University
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
