- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- Expression analysis of estrogen responsiveness in breast cancer cells, 2004
- Creator:
- Halsey, Shalonda B.
- Date of Original:
- 2004-07-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
dissertations - Type:
- Text
- Format:
- application/pdf
- Description:
- Estrogen responsiveness of breast cancers can be associated with the presence or absence of the estrogen receptors (ER). Identification and analysis of estrogen responsive genes is important for proper diagnosis and treatment of estrogen receptor alpha (ERa) negative breast cancer. The objective of this study was to identify differentially expressed genes associated with estrogen treatment of ERa-negative MDA- MB-231 breast cancer cells and ERa-positive 231a breast cancer cells. Total RNA isolated from estrogen treated and untreated cells was subjected to differential microarray examination using the Affymetrix U133A human GeneChip, which consisted of approximately 22,500 genes. Data analyses were performed by Micro array Suite (MAS) 5.0 and GeneSpring 6.0 software analysis systems. Over 10,000 genes and express sequence tags (ESTs) were differentially expressed in estrogen treated cells compared to nontreated cells. There were 529 genes with a 2-fold or higher increase in expression in estrogen treated MDA-MB-231 samples and 231 genes with a 2-fold or higher increase in expression in estrogen treated 231a samples. Genes up-regulated in estrogen treated cells included BRCA1-associated protein 2 (BRAP2), ataxia telangiectasia mutated-Rad3 related (ATR), vascular endothelial growth factor (VEGF) and several ESTs. This study demonstrated that estrogen treatment altered the expression of a diverse group of genes in ERa-negative breast cancer cells, thereby indicating that those genes are activated through mechanisms independent of ERa. The estrogen responsiveness of ERa-negative cells has implications for improved therapeutics of all types of breast cancers.
Date of award: 7/1/2004
Degree type: dissertation
Degree name: Doctor of Philosophy (PhD)
Granting institution: Clark Atlanta University
Advisor: Kimbro, K. Sean - Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:2004_halsey_shalonda_b.pdf
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
