- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- Effects of heat shock on the susceptibility of murine tumor targets to t cell-mediated cytotoxicity, 1999
- Creator:
- Jackson, Kimberly M.
- Date of Original:
- 1999-07-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
dissertations - Type:
- Text
- Format:
- application/pdf
- Description:
- The sensitivity of heat shocked cells to T cell-mediated cytotoxicity was examined in three tumor cell lines, A20 B lymphoma, Yac-1 B lymphoma, and P815 mastocytoma. Tumor cells were subjected to heat shock for 2 hours at 42�C and, then, evaluated at 37�C for sensitivity to lysis by intact allo-specific cytotoxic T lymphocytes (CTLs), perforincontaining granules isolated from CTLs, and Fas-mediated apoptosis. Heat shock at 42�C caused little or no apparent change in sensitivity of all cell lines to lysis by intact CTLs, their isolated cytotoxic granules, and increased slightly to extremely in sensitivity to Fasmediated apoptosis. However, upon altering the temperature by +1�C (43 � 0.1 �C), A20 cells became insensitive to lysis by intact CTLs while remaining sensitive to perforin granules and to Fas-mediated apoptosis. Expression of the inducible heat shock protein (ihsp70) was observed in A20 cells incubated at 43�C, but not in those incubated at 42�C, suggesting a role for heat shock proteins. Furthermore, A20 cells shocked at 43�C did not provoke degranulation and secretion of granzymes by antigen-specific CTLs, although formation of CTL-target conjugates and levels of MHC class I molecules remained unchanged. These observations demonstrate that hyperthermia or febrile conditions may reduce susceptibility of some target cells to CTL attack due to failure of antigen presentation and the inability of CTLs to recognize heat-stressed targets, thus enabling some targets to escape CTL attack. They also suggest that induction of hsp70 may play a role in alterations in antigen presentation.
Degree type: dissertation
Degree name: Doctor of Philosophy (PhD)
Granting institution: Clark Atlanta University
Department: Department of Chemistry
Advisor: Jones, James - Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:1999_jackson_kimberly_m
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
