- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- The role of metabolic activation in the immunosuppressive effects of arylamines and polycyclic aromatic hydrocarbons, 1997
- Creator:
- Lee, Mark E.
- Date of Original:
- 1997-05-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
- Type:
- Text
- Format:
- application/pdf
- Description:
- A mouse model was used to investigate the effect of polycyclic aromatic hydrocarbons (PAH's) and arylamines on the ability of splenocytes to proliferate in vitro. The representative compounds for the PAH's and arylamines are benzo[a]pyrene (BP) and 2-aminofluorene (AF), respectively. Murine derived splenocytes were cultured with the mitogen concanavalin A (ConA) and in the Mixed Lymphocyte Response (MLR) to induce proliferation in the presence of BP, AF, and their metabolites. Studies will be conducted on the ability of the carcinogens and metabolites to perturb the binding capacity and efficiency of ConA. The role of metabolism was explored with an emphasis on P-450IA1 inhibition and induction; correlated with mitogenic responses. The presence of BPDE-DNA adducts was used to prove that BPDE-DNA adducts are associated with BP-associated immunosuppression.
- External Identifiers:
- Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:1997_lee_mark_e
- Rights Holder:
- Clark Atlanta University
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
