- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- Fourier transform infrared spectroscopy study of drug binding to DNA, 1995
- Creator:
- Anokwu, Edward Ekem
- Date of Original:
- 1995-05-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
- Type:
- Text
- Format:
- application/pdf
- Description:
- In this study, observations were made on changes that occurred at the DNA phosphate and base vibrational absorption frequencies, from measurements of infrared spectrum of drug-DNA complexes in aqueous solutions. In the complex spectra of the DNA intercalators, ethidium bromide and copper porphyrin, a shift to lower wavenumber occurred at the in-plane C=0/C=N combination vibrational absorption frequency that appears around 1715 cm'1. The DNA asymmetric phosphate stretching vibrational absorption frequency at 1222 cm * was shifted to a higher frequency. The symmetric vibrational frequency at 1087 cm * was slightly shifted to a lower frequency. These shifts were not observed in the complex spectra of the DNA non-intercalator, vanadyl porphyrin. DNA protein bands can be observed in infrared spectroscopy. These bands diminish or disappear when a sample of DNA undergoes protein extraction. So, by comparing the FT-IR spectra of a DNA sample before and after protein extraction one can definitively determine the extent to which protein has been extracted.
- External Identifiers:
- Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:1995_anokwu_edward_ekem
- Rights Holder:
- Clark Atlanta University
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
