- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- Pinus strobus needle ethanol-soluble proline and ethanol-insoluble protein contents of air and ozone (O2) fumigated new foliage, 1990
- Creator:
- Dlamini, Eunice T.
- Date of Original:
- 1990-07-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
- Type:
- Text
- Format:
- application/pdf
- Description:
- This study determined biochemical changes that occurred in 03- fumigated pine trees. Here are reported ethanol-insoluble, 280 nm contents of charcoal-filtered air- and 03-fumigated (100 and 200 ppb) one yr-old needles from 30 yr-old trees grafted onto two yr-old seedlings. Whereas the A280 nm content of air-fumigated needles was 4.72 + 2.02 mg 0.89 g needle dry wt"1, the contents of 100 and 200 ppb 03-fumigated needles were 16 and 68% less. An absorption spectrum revealed no interference with the quantification of protein which was also detected by colorimetry but < at 280 nm. These results suggest that > 200 ppb 03 is required to significantly depress new foliage's protein content. For proline quantification, ethanol-soluble extracts were dried, reconstituted, spotted onto TLC plates and chromatographed. Subsequent to elution from plates, putative needle proline was quantified by colorimetry. The data indicated that proline could not be effectively quantified by ethanol extraction, TLC purification and either ninhydrin or isatin colorimetry.
- External Identifiers:
- Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:1990_dlamini_eunice_t.pdf
- Rights Holder:
- Clark Atlanta University
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
