- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- The effect of paramorphogenetic agents on wild type and microsclerotial mutants of Verticilliuin dahliae, 1981
- Creator:
- Achudume, Albert C.
- Date of Original:
- 1981-05-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
- Type:
- Text
- Format:
- application/pdf
- Description:
- The morphological and physiological aspects of hyphal growth and development of a wild type strain and several microsclerotia altered mutants of Verticillium dahliae were different for a given paramorphogenetic agent. Paramorphogenetic agents induce morphological and structural changes of the fungus in the laboratory, without any accompanied genetic alterations. Semi�solid and liquid Tolmsoff media were supplemented with either chloramphenicol, sorbose, or 2-deoxy-D-glucose, with the unsupplemented minimum medium being used as the control. Those cultures grown on semi-solid media were used to study the growth pattern, alteration in hyphal morphology, hyphal fusion, pigment formation and frequency of branching. The liquid media were useful for conidial determinations. Chloramphenicol grown cultures produced short hyphae that exhibited an opposite branching pattern. They are also characterized by low percent protein coupled with low dry weight. Sorbose induced thick cell walls and a reticulated pattern of branching, a relatively high dry weight, and low protein content. 2-Deoxy-D-glucose sig�nificantly reduced the growth rate and conidia production. There was also a high percentage of protein accompanied with low dry weight and short branched hyphae. The effect on melanin production was not as significant as it was on other morphological characteristics. These alterations due to paramorphogenetic agents were corre�lated with suspected biochemical alteration and discussed as to their relationship to normal development of the organism.
- External Identifiers:
- Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:1981_achudume_albert_c
- Rights Holder:
- Clark Atlanta University
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
