- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- Evaluation of the enzyme-linked immunosorbent: assay (elisa) test for the detection of leptospiral antibodies in bovine sera, 1979
- Creator:
- Hambie, Edith A.
- Date of Original:
- 1979-08-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
dissertations - Type:
- Text
- Format:
- application/pdf
- Description:
- The Micro-Enzyme-Linked Immunosorbent Assay (ELISA) test was evaluated as a presumptive test for the diagnosis of bovine lepto�spirosis. Disposable flat bottom Micro-ELISA plates were utilized as antigen carriers and test vehicles. The antigen was prepared from a soluble alcohol extract of serovars, hardjo, Hardjoprajitno and ill ini, 3055 and stored at -70 C until ready for use in the test. Serology on each bovine serum sample was performed by using the Microscopic Agglutination Test (MA), the Indirect Hemagglutination Test (IHA), and the Enzyme-Linked Immunosorbent Assay (ELISA) Test. The comparison of the ELISA test with the MA and IHA was done with coded sera, randomized as to order of testing and stored at -20 C. A total of 142 different bovine serum samples was tested for the presence of 1eptospiral antibodies, using antigens serovar hardjo (a pathogen) and serovar ill ini (a saprophyte). Reproducibility was checked by duplicating 83 serum samples and triplicating 58 serum samples, resulting in a final total of 482 bovine serum samples being tested. All sera were tested by MA, IHA, and ELISA tests before being decoded for comparison of results. The total agreement of hardjo sera for both positive and negative sera was 48% among all 3 test procedures, whereas the total agreement of ill ini sera for both positive and negative sera was 92% among all 3 test procedures. The test was safer to perform since there was no need to use live antigens in the test; the test did not require pretreatment of sera; the test was read visually, and the test was a simple and rapid procedure. The sensitivity and specificity appear to have been low; however, in order to obtain further definitive results regarding the specificity, sensitivity, and the role of the ELISA test in the detection of 1eptospiral antibodies in bovine sera, other investigations will be needed in the future.
Date of award: 8/1/1979
Degree type: thesis
Degree name: Master of Science (MS)
Granting institution: Atlanta University
Advisor: Lumb, Judith - Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:1979_hambie_edith_a.pdf
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
