- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- A study of the presence of myosin in the rabbit preimplantation blastocysts and early implanted embryos
- Creator:
- Broadway, Ruby L.
- Date of Original:
- 1979-05-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
- Type:
- Text
- Format:
- application/pdf
- Description:
- A considerable amount of work has been carried out in the in vitro cultivation of the mammalian ova, but little has been done about precursor cells in embryonic development, especially at the blastocyst stage. The purpose of this work was to determine if the presence of specific heart protein, such as cardiac myosin, can be used as a protein marker for the identification or precardiac cells during early blastogenesis in the rabbit embryo. Cardiac myosin, which is normally found in the adult heart of the New Zealand rabbit, has been associated with embryonic heart. Previous studies of skeletal myosin have shown fairly pure extractions. To establish the purity of cardiac myosin polyacrylamide gel electrophoresis was used. There were, however, slow moving bands which were indicative of other proteins (possibly actin and tropomyosin). Guinea pigs were immunized with rabbit cardiac myosin and the antibody present in the serum was tested against purified cardiac myosin using agar gel double diffusion techniques. This serum contained antibody against cardiac myosin from both embryonic and adult hearts. Further studies, using fluorescein-labelled antibody techniques, revealed that cardiac myosin was identifiable in the 10-day-old rabbit embryonic heart, but was not observed in the 6-3/4-day-old blastocysts.
- External Identifiers:
- Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:1979_broadway_ruby_l
- Rights Holder:
- Clark Atlanta University
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
