- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- A method for isolating temperature-sensitive mutants of plasmid DNA, 1977
- Creator:
- Hudson, I. R.
- Date of Original:
- 1977-08-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
- Type:
- Text
- Format:
- application/pdf
- Description:
- The plasmid pMB9 is a Col El derivative and carries re-sistance to tetracycline. This plasmid was used in the iso-lation of seventeen mutants which have been characterized as temperature-sensitive and resistant to tetracycline. These mutants were obtained through in vitro mutagenesis of pMB9 which was returned to an E_. coli host by genetic transforma�tion so that the temperature-sensitive characteristics could be observed. The cells were transformed at a frequency of The plasmid appears to remain relatively stable within the host cell. But covalently closed circular DNA could not be isolated. It is difficult to speculate why problems were encountered in isolating CCC DNA. Further studies need to be done to explain the peculiar nature of these mutants.
- External Identifiers:
- Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:1977_hudson_i_r.pdf
- Rights Holder:
- Clark Atlanta University
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
