- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- Serum composition in the rat during the development of hypertension, 1974
- Creator:
- Aduloju, Amos B.
- Date of Original:
- 1974-05-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
dissertations - Type:
- Text
- Format:
- application/pdf
- Description:
- The primary aim of this study was to determine changes in serum components during the development of high blood pressure (hypertension). One group of young Sprague-Dawley rats (120-160 gm) were made hypertensive by the use of the drug, 5 hydroxytryptamine (5-HT) in con-junction with the hormone, estradiol benzoate. In a second group (150- 165 gm), hypertension was induced by salt-loading after unilateral nephrectomy. The drug treated rats received four intraperitoneal in-jections of 5-HT (50 mg/Kg body weight) in physiological saline and, at the same time, four injections of estradiol benzoate (0.4 ug) in sesame oil at regular intervals. The control rats received four injections of saline and sesame oil. Sodium chloride (1.0%) was made available as drinking water ad libitum. In the salt-loading experiment, the right kidneys were removed and sodium chloride (1.5%) was given to the ex-perimental rats to drink while tap water was given to the controls. Periodic determination of the blood pressure, fluid intake, and urine output showed that both the 5-HT rats and the salt-loaded rats had significant increases in blood pressure, fluid intake, and urine output than control groups. The control groups in both experiments gained more weight than the experimental rats. The 5-HT rats became hypertensive (blood pressure above 150 mm Hg) between the 20th and 45th day while the salt-loaded rats became hypertensive between the 10th and 50th day. Studies on serum chemistry showed that there was no significant difference between the blood urea nitrogen (BUN) and total protein of 5-HT rats and their controls while the salt loaded rats had significantly lower BUN and total protein than the controls. The results of this study show that the 5-HT rats were able to regulate excess saline which enabled some of the serum constituents to remain within normal limits. The results also show that salt-loaded rats can tolerate the development of hypertension by lowering the con�centration of the BUN and total protein thereby decreasing the osmotic pressure and increasing the glomerular filtration in order to eliminate excess saline.
Degree type: thesis
Degree name: Master of Science (MS)
Granting institution: Atlanta University
Department: Department of Biology
Advisor: Myers, Joseph B. - Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:1974_aduloju_amos_b
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
