- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- Some effects of actinomycin D on the development of Rana pjpiens beginning at the feeding stage; a histological study, 1970
- Creator:
- Atkinson, Carl Dewey
- Date of Original:
- 1970-01-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
- Type:
- Text
- Format:
- application/pdf
- Description:
- A study of the effects of actinomycin D (an inhibitor of DNA-primed RNA synthesis) on Rana plplens development beginning at the feeding stage (stage 25) was undertaken. Controls were cultured in a 10 Holtfreter's saline solution and experimentals in a Holtfreter-actinomycin D (10Ag/ml) solution for 10, 20, and 36 hr. Histological analysis disclosed abnormal differentiation of the tail notochord, brain, and spinal cord in the 20 and 36 hr treated larvae. The 10 hr treated larvae showed no distinct cellular changes from their corresponding controls. The brain, spinal cord, and notochord of the controls differentiated normally. It is apparent from this investigation that actinomycin D exerts morphostatic effects upon Rana plpiens larvae at the feeding stage. Similar to previous work using earlier stages, the antibiotic strongly affected neural tissue differentiation.
- External Identifiers:
- Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:1970_atkinson_carl_dewey
- Rights Holder:
- Clark Atlanta University
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
