- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- A study of the adhesive properties of notochordal cells in larval amphibians, 1969
- Creator:
- C, Joyce Beavers
- Date of Original:
- 1969-06-01
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
- Type:
- Text
- Format:
- application/pdf
- Description:
- This study was undertaken to examine the adhesive forces of notochordal cells in larval amphibians. Notochords were dissected from tadpoles and incubated in a selected group of enzymes and a chelating agent to determine the effectiveness of each in dissociating this tissue into individual cells. Notochordal fragments incubated in preparations of papain, pepsin, and hyaluronidase made up as 3% solutions in calcium and magnesium free Holtfreter's solution, a 3% solution of trypsin in distilled water, and a 2% ethylenediaminetetraacetic acid calcium and magnesium free Holtfreter's solution were completely dissociated into suspensions of vacuolated cells within 1 hr. Individual peripheral cells were observed in suspension only from fragments which were incubated in papain and hyaluronidase preparations. Fibrous structures and other extracellular materials were observed in all suspensions. It was concluded in light of these observations that a cement or ground substance is involved in cell adhesion in the larval amphibian notochord. Also, that calcium and/or magnesium are involved in the stabili-zation of this ground substance.
- External Identifiers:
- Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:1969_c_joyce_beavers.pdf
- Rights Holder:
- Clark Atlanta University
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
