A Modified Coupled Spectrophotometric Method to Detect 2-5 Oligoadenylate Synthetase Activity in Prostate Cell Lines

Collection:
Clark Atlanta University Faculty Publications
Title:
A Modified Coupled Spectrophotometric Method to Detect 2-5 Oligoadenylate Synthetase Activity in Prostate Cell Lines
Creator:
Bhosle, Sushma M.
Hunt, Aisha
Chaudhary, Jaideep
Date of Original:
2016-01-01
Subject:
African Americans--Education (Higher)--Georgia
Clark Atlanta University
Location:
United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
Medium:
articles
Type:
Text
Format:
application/pdf
Description:
Abstract: Background: 2-5 oligoadenylate synthetases (OAS) are interferon inducible enzymes that polymerizes ATP to 2-5-linked oligomers of adenylate (2-5As). As part of the innate immune response, these enzymes are activated by viral double stranded RNA or mRNAs with significant double stranded structure. The 2-5As in turn activate RNaseL that degrade single stranded RNAs. Three distinct forms of OAS exist in human cells (OAS1, 2 and 3) with each form having multiple spliced variants. The OAS enzymes and their spliced variants have different enzyme activities. OAS enzymes also play a significant role in regulating multiple cellular processes such as proliferation and apoptosis. Moreover, Single nucleotide polymorphisms that alter OAS activity are also associated with viral infection, diabetes and cancer. Thus detection of OAS enzyme activity with a simple spectrophotometric method in cells will be important in clinical research.
Source: Biological Procedures Online 18, no. 9 (2016)
DOI: 10.1186/s12575-016-0038-x.
URL: http://dx.doi.org/10.1186/s12575-016-0038-x.
Metadata URL:
http://hdl.handle.net/20.500.12322/cau.ir:2016_bhosle_sushma
Language:
eng
Original Collection:
Clark Atlanta University Faculty Publications
Holding Institution:
Clark Atlanta University
Rights:

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