- Collection:
- Atlanta University and Clark Atlanta University Theses and Dissertations
- Title:
- Solubility Ratios, Encapsulation Efficiency, and Size of Beta-sitosterol Loaded Poly(Lactide)-Block-Poly(Ethylene glycol) Polymeric Micelles, 2019
- Creator:
- Alqarni, Ali
- Date of Original:
- 2019-07-31
- Subject:
- Degrees, Academic
Dissertations, Academic - Location:
- United States, Georgia, Fulton County, Atlanta, 33.749, -84.38798
- Medium:
- theses
dissertations - Type:
- Text
- Format:
- application/pdf
- Description:
- B-sitosterol/poly(ethylene glycol)-block-poly(lactic acid) (PLA-b-PEG) complexes were prepared by solution blending in purified water and ethanol. The mixture of water and ethanol is a suitable solvent system for the two components. The complex was studied by using Nuclear Magnetic Resonance (NMR) spectroscopy and Differential Scanning Calorimetry (DSC). B-sitosterol is a drug that may reduce the swelling of benign prostatic hyperplasia (BPH) and diminishing inflammation. However, it is hydrophobic and difficult to deliver in aqueous solution. Since PLA-b-PEG has amphiphilic properties, the complex described here may enhance delivery of this drug for treatment of BPH. Proton NMR (1HNMR) of the complexes shows that the methylene (CH2) protons of the PEG, the (-O-CH-) of PLA, and (CH3) of PLA are slightly shifted because of its non-covalent interaction with B-sitosterol. The complex formation was supported by 2-D NMR (NOESY) spectroscopy. NOESY spectra show cross peaks, indicating the interaction between the two components. DSC of the complexes shows thermal characteristics that are different from the individual components. In particular, the PEG in the complex shows a lower melting point and decreased crystallinity compared to the pure PEG. The melting point is lowered from 57C to 55.3C for the PEG-b-PLA/?-sitosterol (5%) complex. Under the same condition, the melting point of PLA dropped from 170C to 130C. Atomic force microscopy shows changes in the surface morphology of the copolymer from crystalline to amorphous when incorporated with the drug. NMR, DSC, AFM, and MTT assay studies suggest the formation of a relatively stable ?-sitosterol/poly(lactic acid)-block-poly(ethylene glycol) complex. The cell proliferation assay (MTT assay) suggests significant inhibition of the stimulation of growth of prostate cancer cells upon addition the complex.
Date of award: 7/31/2019
Degree type: dissertation
Degree name: Doctor of Philosophy (PhD)
Granting institution: Clark Atlanta University
Department: Chemistry
Advisor: Khan, Ishrat
Advisor: Parker,Cass
Advisor: Wang, Xiao-Qian - Metadata URL:
- http://hdl.handle.net/20.500.12322/cau.td:2019_alqarni_ali
- Holding Institution:
- Atlanta University Center Robert W. Woodruff Library
- Rights:
-
